human es cell lines skes1, rdes, sknmc and scch  (JCRB Cell Bank)

Journal: Journal of Pediatric Hematology/Oncology

Article Title: Augmented Antitumor Activity for Novel Dual PI3K/BDR4 Inhibitors, SF2523 and SF1126 in Ewing Sarcoma

doi: 10.1097/mph.0000000000002054

Figure Lengend Snippet: FIGURE 2. SF1126 and SF2523 decreases cell viability and phosphorylation of AKT in Ewing sarcoma cell lines. A, IC50 curves of the 5 ES cell lines treated with SF1126, SF2523, and SF2523P. B, WB results for A673, EWS502, SK-N-MC, SK-PN-DW, and RDES treated with different concentrations of SF1126 followed by lysate preparation after 30 minutes of treatment and Western blot analysis. C, WB results for A673, EWS502, SK-N-MC, and SK-PN, DW treated with various doses of SF2523 for 30 minutes followed by lysate preparation and western blot. D, WB results for A673 and EWS502 with 30 minutes of SF2523P treatment at 0.1, 1, and 5 µM concentrations. WB indicates western blot.

Article Snippet: A673, EWS502, SK-N-MC, SK-PN-DW, and RDES human cell lines were obtained from the ATCC collection and provided by Sun Choo, M.D., UC San Diego Department of Pediatrics.

Techniques: Phospho-proteomics, Western Blot

Journal: PLoS ONE

Article Title: c-Myc Represses Tumor-Suppressive microRNAs, let-7a, miR-16 and miR-29b, and Induces Cyclin D2-Mediated Cell Proliferation in Ewing’s Sarcoma Cell Line

doi: 10.1371/journal.pone.0138560

Figure Lengend Snippet: (A), Transfection of c-Myc siRNA in SKES1 reduces the expression of c-Myc protein. (B), The quantification of c-Myc protein after transfection of c-Myc siRNA. (C), miRNA quantification of let-7a, miR-16 and miR-29b expression after transfection with c-Myc siRNA by quantitative RT-PCR. (D-F,) After actinomycinD treatment, the miRNA expression level of let-7a, miR-16 and miR-29b in the negative control-miR, let-7a, miR-16, miR-29b and their mutant was measured by qRT-PCR. After actinomycinD treatment, the mRNA expression level of CCND2 after transfection of negative control-miR, let-7a, miR-16, miR-29b and their mutant was measured by qRT-PCR. ANOVA was performed to statistically analyze the data. * p < 0.05 and ** p < 0.01.

Article Snippet: The human ES cell lines, SKES1, RDES, SKNMC and SCCH were obtained from JCRB Cell Bank (Tokyo, Japan), and WE68 was kindly provided Dr. Frans van Valen (Westfalische-Wilhelms University, Munster, Germany).

Techniques: Transfection, Expressing, Quantitative RT-PCR, Negative Control, Mutagenesis

Journal: PLoS ONE

Article Title: c-Myc Represses Tumor-Suppressive microRNAs, let-7a, miR-16 and miR-29b, and Induces Cyclin D2-Mediated Cell Proliferation in Ewing’s Sarcoma Cell Line

doi: 10.1371/journal.pone.0138560

Figure Lengend Snippet: The expression of CCND2 protein was decreased in SKES1 transfected with let-7a (A), miR-16 (C), miR-29b (E) and CCND2-siRNA (G). Densitometry quantification of CCND2 protein levels after transfection of let-7a (B), miR-16 (D), miR-29b (F) and CCND2-siRNA (H). * p < 0.05 and ** p < 0.01.

Article Snippet: The human ES cell lines, SKES1, RDES, SKNMC and SCCH were obtained from JCRB Cell Bank (Tokyo, Japan), and WE68 was kindly provided Dr. Frans van Valen (Westfalische-Wilhelms University, Munster, Germany).

Techniques: Expressing, Transfection

Journal: PLoS ONE

Article Title: c-Myc Represses Tumor-Suppressive microRNAs, let-7a, miR-16 and miR-29b, and Induces Cyclin D2-Mediated Cell Proliferation in Ewing’s Sarcoma Cell Line

doi: 10.1371/journal.pone.0138560

Figure Lengend Snippet: The SKES1 were treated and analyzed by flow cytometry after PI staining (A–E). Histogram shows the quantitative percentage of diploid cells (DNA content) in each cell-cycle phase (F). Effects of let-7a, miR-16 and miR-29b on the induction of apoptosis in SKES1 cells. Western blotting shows the expression of PARP (G) and its cleaved form (H). The cells were labeled with FITC annexin V and PI (I–N). Each quadrants represent viable cells (Lower Left quadrant), early apoptotic cells (Lower Right), late or secondary necrotic cells (Upper Right), and primary necrotic cells (Upper Left), respectively. * p < 0.05.

Article Snippet: The human ES cell lines, SKES1, RDES, SKNMC and SCCH were obtained from JCRB Cell Bank (Tokyo, Japan), and WE68 was kindly provided Dr. Frans van Valen (Westfalische-Wilhelms University, Munster, Germany).

Techniques: Flow Cytometry, Staining, Western Blot, Expressing, Labeling

Journal: PLoS ONE

Article Title: c-Myc Represses Tumor-Suppressive microRNAs, let-7a, miR-16 and miR-29b, and Induces Cyclin D2-Mediated Cell Proliferation in Ewing’s Sarcoma Cell Line

doi: 10.1371/journal.pone.0138560

Figure Lengend Snippet: (A), Transfection of c-Myc siRNA in SKES1 reduced the expression of CCND2 protein. (B), Transfection of CCND2 siRNA in ES cells reduced the expression of CCND2 protein. The cells were treated and analyzed by flow cytometry after PI staining (C–F). Histogram shows the quantitative percentage of diploid cells (DNA content) in each cell-cycle phase in c-Myc and CCND2-siRNA treated cells (G). Effects of c-Myc and CCND2-siRNA on induction of apoptosis in the SKES1 cells. The cells were labeled with FITC annexin V and PI (H–L). * p < 0.05.

Article Snippet: The human ES cell lines, SKES1, RDES, SKNMC and SCCH were obtained from JCRB Cell Bank (Tokyo, Japan), and WE68 was kindly provided Dr. Frans van Valen (Westfalische-Wilhelms University, Munster, Germany).

Techniques: Transfection, Expressing, Flow Cytometry, Staining, Labeling